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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 9 Documents
 1 
Search results for , issue "Vol 10 No 4 (2009)" : 9 Documents clear
Deteksi Produksi Toksin Stx-1 dan Stx-2 dari Escherichia coli O157:H7 Isolat Lokal Hasil Isolasi Feses dan Daging Sapi I Wayan Suardana; I Gusti Made Krisna Erawan; Bambang Sumiarto; Denny Widaya Lukman
Jurnal Veteriner Vol 10 No 4 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Shiga toxin produced by Escherichia coli O157:H7 can cause outbreaks and sporadic cases of serioushuman diseases. The diseases are indicated by hemorrhagic colitis and hemolytic uremic syndrome. Meatand meat products have been identified as vehicles of food borne disease caused by E.coli O157:H7. Themain aim of this research was to identify the correlation between the level of E.coli O157:H7 contaminationand the presence of Shiga toxin (Stx1 and Stx2) by applying method of Vero toxin Escherichia coli-ReversePassive Agglutination Test (VTEC-RPLA). The results showed that 3 of 7 isolates and 1 of 4 isolatesisolated from feces of cattle and beef, respectively produced Stx 1 (VT1). In the detection of Stx 2 (VT2), 4of 7 isolates and 1 of 4 isolates, isolated from the same samples were found to produce this toxin.According to all isolates, in this research showed, 1 isolate was found to produce VT2, 4 isolates to produceboth VT1 and VT2, while 6 isolates showed negative results either to VT1 or VT2.
PERUBAHAN VIABILITAS DAN STRUKTUR SUBSELULER SPERMATOZOA DOMBA SETELAH PENGERINGBEKUAN Takdir Saili; I Ketut Mudite Adnyana; Ronny Rachman Noor; Mohamad Agus Setiadi; Srihadi Agungpriyono; Arief Boediono
Jurnal Veteriner Vol 10 No 4 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Several methods i.e. cooling, freezing, and freeze-drying have been widely used to preserve spermatozoa with various degree of success. Freeze-drying appears to provide a method to preserve spermatozoa in a dry state without requiring liquid nitrogen for storing frozen spermatozoa. Freeze-drying procedures can have a detrimental effect on plasma membrane and acrosomal cap of the spermatozoa. In this experiment study, the viability and subcellular changes of freeze-dried ram spermatozoa were evaluated using staining method and scanning electron microscopy. The results revealed that all freeze-dried spermatozoa were dead following evaluation using eosin staining and Hoechst-propidium iodide staining methods. Morover, plasma membrane and acrosomal cap of freeze-dried ram spermatozoa was disrupted observed using scanning electron microscope.
Patogenisitas Streptokokus Grup B pada Mencit Neonatus Zinatul HayatiO; Teuku Fadrial Karmil
Jurnal Veteriner Vol 10 No 4 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Group B Streptococci (GBS) are the major cause of serious infections in neonates, including pneumonia,septicemia and meningitis. Considering that GBS bacteria founded in Indonesia in our previous study isdissimilar as what have been found in foreign country particularly in its serotype distribution, thereforethis research is extremely essential to be conducted in Indonesia in order to searching the vaccine candidateto be used in Indonesia. This research focused in pathogenicity test of each GBS isolates on animal model.Pathogenicity experiment has been done by early onset septicemia model approach to neonatal mouse.Pathogenicity test result that SR-7 isolates can cause the death of neonatal mouse 100% in early-onsetinfection. Whereas, SV-2 isolates cannot cause the neonatal mouse neither in early-onset nor in late-onset.From the serotype determination result at previous research found that SR-7 isolates is serotype isolatesVI whilst SV-2 isolates is serotype III. This result has shown that SR-7 (serotype VI) isolates is the mostpathogen and the SV-2 isolates is less pathogen.
Bioaktivitas Forbazol-E terhadap Kerusakan Ultrastruktur Dinding Sel Staphylococcus aureus Ni Putu Ristiati; Ketut Suata; Dewa Ngurah Suprapta
Jurnal Veteriner Vol 10 No 4 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The bioactivity role of phorbazol-E in inhibiting bacterial protein synthesis is unknown. Onthe contrary, phorbazol-A, B, C, and D have been proved to inhibit the bacterial protein synthesis.The effect of forbazol-E on Staphylococcus aureus ATCC25922 cell wall ultrasctucture destructionwas observed. The study used randomized-post test-only control group design which consist of threetreatment (control, treatment I and II) with 9 repeatation. Treatment I: 1 ml overnight culture ofS. aureus was cultured into a mixed of 20 ml Mueller Hinton (MH) broth and 37.5 mg/L phorbazol-E; whereas in treatment II phorbazol-E used was 75.0mg/L. The growth curve of S. aureus wasmonitored using spectrophotometer; whilst the destruction of cell wall ultrastructure was observedusing Transmission Electron Microscope (TEM) at Eijkman Insitute, Jakarta. Phorbazol-E at75.0mg/L significantly caused the destruction of the bacteria cell wall ultrastructure and inhibitedthe bacteria growth in comparison to phorbazol-E at 37.5 mg/L (p<0.05).
Experimental Infection of Taenia saginata eggs in Bali Cattle: Distribution and Density of Cysticercus bovis Nyoman Sadra Dharmawan; I Made Damriyasa; I Nengah Kapti; Putu Sutisna; Munehiro Okamoto; Akira Ito
Jurnal Veteriner Vol 10 No 4 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this study was to observe the development, distribution, and infection density ofTaenia saginata metacestodes in Bali cattle. Three Bali cattle were experimentally infected with T. saginataeggs which were collected from taeniasis patients. The experimental animal was inoculated with : i)1000,00 T. saginata; ii) 500,000 eggs; and iii) 1,000,000 eggs, respectivelly 100,000 (cattle 1), 500,000(cattle 2), and 1,000,000 (cattle 3) T. saginata eggs, respectively. To observe the development of cysticerci,all cattle were slaughtered at 24 weeks post infection. To observe their distribution and density, slicingwas done to the cattle?s tissues. The study results showed that cysts were found distributed to all muscletissues and some visceral organs such as heart, diaphragm, lungs, and kidney of the cattle infected with100,000 and 500,000 T. saginata eggs. Density of the cyst was in the range of 11 to 95 cysts per 100 gramsof tissue. The highest density was noted in the heart (58/100 grams) and in diaphragm (55/100 grams).This study has confirmed that T. saginata eggs derived from taeniasis patient in Bali, if infected to Balicattle can develop and spread to all muscle tissues and some visceral organs. From this study it wasconcluded that it is necessary to include the heart in the meat inspection at slaughter house for possibilityof T. saginata cyst infection.$?
Pemilihan Tempat Bertelur Nyamuk Aedes aegypti pada Air Limbah Rumah Tangga di Laboratorium I Made Sudarmaja; SugengO Juwono Mardihusodo
Jurnal Veteriner Vol 10 No 4 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A laboratory experimental study was conducted to observe the types of household waste water aspreferential sites for Aedes aegypti to lay their eggs. In this study, three types of waste water were used i.e.soap contaminated water, detergent contaminated water and tap water respectively. The results of thestudy showed that soap contaminated water (0,5 gram/liter) and tap water were preferential sites forAedes aegypti to lay their eggs, while detergent contaminated water was not. The number of mosquito eggsfound in soap contaminated water was not significantly different with those found in tap water but both ofthem were significantly higher than those found in detergent contaminated water.
PERUBAHAN VIABILITAS DAN STRUKTUR SUBSELULER SPERMATOZOA DOMBA SETELAH PENGERINGBEKUAN Takdir Saili; I Ketut Mudite Adnyana; Ronny Rachman Noor; Mohamad Agus Setiadi; Srihadi Agungpriyono; Arief Boediono
Jurnal Veteriner Vol 10 No 4 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Several methods i.e. cooling, freezing, and freeze-drying have been widely used to preserve spermatozoa with various degree of success. Freeze-drying appears to provide a method to preserve spermatozoa in a dry state without requiring liquid nitrogen for storing frozen spermatozoa. Freeze-drying procedures can have a detrimental effect on plasma membrane and acrosomal cap of the spermatozoa. In this experiment study, the viability and subcellular changes of freeze-dried ram spermatozoa were evaluated using staining method and scanning electron microscopy. The results revealed that all freeze-dried spermatozoa were dead following evaluation using eosin staining and Hoechst-propidium iodide staining methods. Morover, plasma membrane and acrosomal cap of freeze-dried ram spermatozoa was disrupted observed using scanning electron microscope.
Tingkat Maturasi in vitro Oosit Kambing dalam Medium dengan Suplementasi Serum dan Albumin Sri Gustari; Ni Wayan Kurniani Karja; Yuke Rizky Amelia; Ian Kurniawan; Bayu Sulistyo
Jurnal Veteriner Vol 10 No 4 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The present study aimed to study the effect of ?maturation media? on maturation rate of goat oocytesafter in vitro maturation. Goat ovaries were collected from a slaughter house in Godean, Sleman. Immediatelyafter slaughter the ovaries were collected, rinsed with physiological NaCl three times then placed in aflask containing the NaCl solutions and hept at 36-370C before transportaion to the laboratory. Oocyteswere observed under stereo microscope and its quality was classified into A, B, and C. Oocytes in vitromaturaion (IVM) was performed in TCM-199 media suplemented with : a) 0.4 mg/ml bovine serum albumin(BSA); and b) 10% newborn calf serum (NCS) then incubated at 38.50C with 5% CO2 for 24-27 h. Followingthis, oocytes were observed under inverted microscope for first polar body extrusion, then stained withaceto-orcein in order to evaluate nuck or maturation. The nuclear maturation stages including : germinalvesicles (GV), germinal vesicles break down (GVBD), metaphase I, apaphase I, telophase I and metaphaseII, respectivelly. The overall results showed that 74-74%, 52-66.6% and 21.5-23.8% of oocytes quality A, B,and C reached maturation at metaphase II, respectivelly. There were no significant differences in oocytesmaturation using media supplemented with either BSA or NCS.
Vitrifikasi Blastosis Mencit dengan Metode Kriolupv ?O I Wayan Batan; I Ketut Suatha; Wahono Esti PrasetyoningtyaserB; Nining Handayani; Ita Djuwita; Arief Boediono
Jurnal Veteriner Vol 10 No 4 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Cryopreservation is an ultra rapid freezing process to preserve tissue or organ. The studywas conducted to identify the effectiveness of cryoloop vitrification method and the viability ofembryos following vitrification. Embryos at blastocyst stage were vitrified by placing them inequilibration medium containing 10% ethylene glycol (EG) and phosphate buffer saline (PBS) wichsupplemented with 20% new born calf serum for 8-10 minutes. The blastocysts were then removedand put in vitrification medium (15% dimethyl sulfoxide, 15% EG, and 0.5M sucrose), and theprocess in the vitrifivcation medium not longer than 25-30 seconds. The blastocysts were immediatelytransferred to the vitrification medium film in the cryoloop and plunged into 100 ml liquid nitrogen.The warming process was done by immersing the cryoloop which carried the vitrified blastocstsinto PBS supplemented with 20% serum and 0.5M sucrose for 1 minute, and then removed to samesolution supplemented with 0.25M sucrose and 0.1M sucrose for 2 minutes respectively. Theblastocysts were washed 4 times in kalium simplex optimized medium (KSOM) and cultured indrops of KSOM in 5% CO2 incubator at 370C. The observations were done every 6 hours for 48hours using inverted microscope ( Olympus IX70 Japan). The viability of embryos was assessed onthe basis of the intact morphology, reexpansion of the blastosul, and the development of embryosinto advance stage. The results showed that 85.71% of vitrified embryos, developed into advancestages and 19% of them hatched. In conclusion the cryoloop can be used to vitrify the embryos.

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